Hydrophobic Cu12Sb4S13 nanoparticles with strong and full-spectrum photoabsorption in the range of 200–2500 nm were fabricated and deposited on cellulose acetate (CA) membrane to form a photothermal film, which could realize photothermal heating for vapor generation and thermodestruction of pathogenic bacteria of E. coli O157:H7 simultaneously under light irradiation.