DNA nanostructure-based thermometers were fabricated by linking silver nanoclusters (AgNCs) and guanine-rich（G-rich）DNA chains via a thermally sensitive DNA stem-loop at terminals 5' and 3'. Variations of alter the distance between the AgNCs and G-rich DNA chain, affecting the interaction between them. As a result, the intensity of from the AgNCs at 636 nm can be sensitively modulated. It was found that the intensity of such red is more sensitive than the equivalent green at 543 nm; sensitivity of −3.6%/°C was achieved. Through variation of the melting of the DNA stem-loop, the response range of the thermometers could be readily adjusted. Novel DNA nanostructure-based thermometers as described in this work are anticipated to be able to measure the of biological systems at small scales—even a .